Name and publication

Phytophthora intricata T. Jung, M. Horta Jung, Scanu & Bakonyi (2017)

Jung T, Jung MH, Scanu B, Seress D, Kovács GM, Maia C, Pérez-Sierra A, Chang T-T, Chandelier A, Heungens K, van Poucke K, Abad-Campos P, Léon M, Cacciola SO, and Bakonyi J. 2017. Six new Phytophthora species from ITS Cladeclade:
a taxonomic group of organisms classified together on the basis of homologous features traced to a common ancestor
7a including two sexually functional heterothallicheterothallic:
pertaining to sexual reproduction in which conjugation is possible only through interaction of different thalli (i.e. different mating types) (cf. homothallic)
hybrid species detected in natural ecosystems in Taiwan. Persoonia 38: 100–135.

Corresponding author: trjung@ualg.pt, thomas.jung@mendelu.cz

Nomenclature

from Jung et al. (2017)

Mycobank

MB816569

Etymology

refers to the intricate, intertwining oogonial and antheridial stalks (intricata Lat = intricate or intertwining)

Typification

Type: TAIWAN, Fushan, isolated from rhizosphere soil of a planted Quercus tarokoensis tree, T. Jung, 2013 (CBS H-22553 holotype, dried culture on V8A, Herbarium CBS-KNAW Fungal Biodiversity Centre)

Ex-type: CBS 141211 = TW259 

Sequences for ex-type in original manuscript: Phytophthora intricata isolate TW259 = ITS rDNA KU517155, cox1 KU517149

Ex-type in other collections

(ET) CBS 141211, NRRL 64321, TW259, 67B9 (Hong)

Molecular identification

Voucher sequences for barcoding genes (ITS rDNA and COI) of the ex-type (see Molecular protocols page)

Phytophthora ipomoeae isolate CPHST BL 21 (= P10225 WPC) = ITS rDNA MG865518, COI MH136912

Voucher sequences for Molecular Toolbox with seven genes (ITS, β-tub, COI, EF1α, HSP90, L10, and YPT1

(see Molecular protocols page) (In Progress)

Voucher sequences for Metabarcoding High-throughput Sequencing (HTS) Technologies [Molecular Operational Taxonomic Unit (MOTU)]

(see Molecular protocols page) (In Progress)

Sequences with multiple genes for ex-type in other sources

• NCBI: Phytophthora ipomoeae CPHST BL 21 
• NCBI: Phytophthora ipomoea P10225
• NCBI: Phytophthora ipomoeae PD-00078 (P10225)
• NCBI: Phytophthora ipomoea CBS 109229 ITS in 2013 manuscript
• EPPO-Q-bank: Phytophthora ipomoea CBS 109229
• BOLDSYSTEMS: Phytophthora ipomoeae PHYTO022-10 (= P10225) (barcoding COI & ITS)

Position in multigenic phylogeny with 7 genes (ITS, β-tub, COI, EF1α, HSP90, L10, and YPT1)

Clade clade:
a taxonomic group of organisms classified together on the basis of homologous features traced to a common ancestor
1c

Morphological identification

adapted from Jung et al. (2017)

Colonies and cardinal temperatures

Colony colony:
assemblage of hyphae which usually develops form a single source and grows in a coordinated way
morphology on V8A uniform, on MEA faintly stellate, and on PDA rosaceous. Minimum growth temperature 5 °C, optimum 30 °C, and maximum 35 °C.

Conditions for growth and sporulation

Sporangia not formed on solid agar but were produced abundantly in non-sterile soil extract. GametangiaGametangia:
part of hypha specialized for fusion in sexual reproduction (see antheridium and oogonium)
readily produced on V8A in single culture.

Asexual phase

SporangiaSporangia:
sac within which zoospores form, especially when water is cooled to about 10°C below ambient temperature; in solid substrates, sporangia usually germinate by germ tubes
nonpapillatenonpapillate:
pertaining to the production of a non-distinct, or inconspicuous, papilla at the distal end of the sporangium (cf. papillate and semipapillate)
; persistentpersistent:
pertaining to sporangia that remain attached to the sporangiophore and do not separate or detach easily (cf. caducous)
; ovoidovoid:
egg-shaped, with the widest part at the base of the sporangium and the narrow part at the apex
, elongated, obpyriformobpyriform:
inversely pear-shaped, i.e. with the widest part at the point of attachment (cf. pyriform)
, limoniform, ellipsoidellipsoid:
refers to a solid body that forms an ellipse in the longitudinal plane and a circle in cross section; many fungal spores are ellipsoidal or elliptic
to less frequently pyriformpyriform:
pear-shaped, with the narrowest part at the base (cf. obpyriform)
, subglobose, ampulliform, bilobed or trilobed (39–78.9 μm length × 24.1–48.8 μm width); presenting internal (nested) and extended proliferationextended proliferation:
a type of internal proliferation in which the sporangiophore originates from inside of an empty sporangium, and continues to grow through and out of the old sporangium
; originated on unbranched sporangiophores or in lax sympodiasympodia:
a type of sporangiophore which appears simple, but where each successive sporangium develops on a branch behind and to one side of the previous apex, where growth has already ceased
. Hyphal swellings absent. ChlamydosporesChlamydospores:
an asexual spore with a thickened inner wall that is delimited from the mycelium by a septum; may be terminal or intercalary, and survives for long periods in soil
absent.

Sexual phase

Homothallic. Oogonia smooth-walled, globoseglobose:
having a rounded form resembling that of a sphere
to subglobose (26.3–56.7 μm diam.) or slightly eccentric with a widened base between the oogonial stalk and the point of antheridial attachment; antheridiaantheridia:
the male gametangium; a multinucleate, swollen hyphal tip affixed firmly to the wall of the female gametangium (the oogonium)
paragynousparagynous:
pertaining to the sexual stage in which the antheridium is attached to the side of the oogonium (cf. amphigynous)
, subglobose to cylindrical, with antheridial stalks often twisted and intertwining; oosporesoospores:
zygote or thick-walled spore that forms within the oogonium after fertilization by the antheridium; may be long-lived
pleroticplerotic:
pertaining to an oospore that fills the oogonium (cf. aplerotic)
(25.1–50.3 μm diam.), thick-walled, and contain two nuclei and a large ooplast.

Hosts and distribution

Distribution: Asia (Taiwan)
Substrate: rhizosphere soil
Host: Quercus tarokoensis (Fagaceae)

Retrieved January 31, 2018 from U.S. National Fungus Collections Nomenclature Database.

Additional references and links

 

• SMML USDA-ARS: Phytophthora intricata
• EPPO Global Database: Phytophthora intricata
• Forest Phytophthora of the world: Phytophthora intricata
• CABI Digital Library: Phytophthora intricata
• Encyclopedia of Life (EOL): Phytophthora intricata
• Index Fungorum (IF): Phytophthora intricata

 

• Google All Phytophthora intricata
• Google Images Phytophthora intricata
• Google Scholar Phytophthora intricata

Fact sheet author

Z. Gloria Abad, Ph.D., USDA-APHIS-PPQ-S&T Plant Pathogen Confirmatory Diagnostics Laboratory (PPCDL), United States of America.