Phytophthora bilorbang

Name and publication

Phytophthora bilorbang Aghighi & Burgess (2012)

Aghighi S, Hardy GE StJ, Scott JK, and Burgess TI. 2012. Phytophthora bilorbang sp. nov., a new species associated with the decline of Rubus anglocandicans (European blackberry) in Western Australia. Eur. J. Plant Pathol. 133: 841–855.

Corresponding author: tburgess@murdoch.edu.au

Nomenclature

from Aghighi et al. (2012)

Mycobank

MB563863

Etymology

refers to a Noongar (southwestern Australian Aboriginal) word for a person living on the banks of a river

Typification

Type: WESTERN AUSTRALIA, Pemberton, banks of Warren River, from rhizosphere soil of dying Rubus anglocandicans, collected by S. Aghighi in 2010, holotypus MURU 470 (dried culture on V8A, Herbarium of Murdoch University, Western Australia)

Ex-type: CBS 131653 = SA262

Sequences for ex-type in original manuscript: Phytophthora bilorbang strain CBS 131653 = ITS rDNA= JQ256377, coxI = JQ256375, HSP90 = JQ256376, B-tub = JQ256374 and NADH = JQ256378

Ex-type in other collections

P19863 (WOC/WPC), CPHST BL 101 (Abad), 61G8 (Yang)

Molecular identification

Voucher sequences for barcoding genes (ITS rDNA and COI) of the ex-type (see Molecular protocols page)

Phytophthora bilorbang isolate CBS 161653 ITS rDNA JQ256377

Phytophthora bilorbang isolate CPHST BL 101 COI MH477742

Sequences for ex-type in other sources
Position in ITS phylogenetic tree

Clade 6b

Morphological identification

Colonies and cardinal temperatures

Colony morphology is stellate to petaloid on V8A, sparse on CA and MEA, and dense/felty and petaloid on PDA. Minimum growth temperature 4°C, optimum 25°C, and maximum 32.5°C.

Conditions for growth and sporulation

Sporangia are produced on solid media and in water cultures (soil extract or river water). Oogonia are formed readily in single-strain culture on CA and V8A after about 20 d.

Asexual phase

Sporangia are nonpapillate, persistent, and limoniform, ovoid, or ellipsoid in shape. Sporangia average 51.6 ± 6.4 x 29.0 ± 4.6 µm (overall range 22.8–80.7 x 11.7–40.4 µm). Sporangiophores usually in simple sympodia with internal proliferation, both nested and extended. External proliferation also observed leading to lax sympodia. Hyphal swellings are common and globose to ellipsoid, catenulate, or angular with radiating hyphae. Chlamydospores absent.

Sexual phase

Homothallic. Oogonia are globose, average size 33.9 ± 6.0 mm (19.6–47.3 µm) Oospores are plerotic to slightly aplerotic, globose with a large ooplast, or commonly with two ooplasts and multiple nuclei, average size 32.2 ± 6.1 µm (18.1–44.7 µm ). Antheridia are paragynous often with several finger-like projections

Most typical characters

Phytophthora bilorbang closely resembles other homothallic Clade 6 species.

Specimen(s) evaluated

Australia, Western Australia, Pemberton, banks of the Warren River, from rhizosphere soil of dying Rubus anglocandicans, 2010, collected by S. Aghighi, CBS 131653 = SA262; SA92, SA142, SA143, SA146

CPHST BL 101 (ET) = P19863 World Oomycetes /Phytophthora Collection – California, USA

Hosts and distribution

Distribution: Western Australia
Substrate: roots, collars and rhizosphere soil
Disease note: pathogenic toward Rubus anglocandicans
Hosts: Rubus anglocandicans

Retrieved January 19, 2018 from U.S. National Fungus Collections Nomenclature Database.

Additional references and links

Burgess TI, Webster JL, Ciampini JA, White DW, Hardy GESJ, Stukely MJC. 2009. Re-evaluation of Phytophthora species isolated during 30 years of vegetation health surveys in Western Australia using molecular techniques. Plant Disease 93, 215-223.

Jung T., Stukely M. J. C., Hardy G. E., White D., Paap T., Burgess T. I. 2011. Multiple new Phytophthora species from ITS Clade 6 associated with natural ecosystems in Australia: evolutionary and ecological implications. Persoonia 26: 13–39.

Brasier CM, Cooke DEL, Duncan JM, Hansen EM. 2003a. Multiple new phenotypic taxa from trees and riparian ecosystems in Phytophthora gonapodyides – P. megasperma ITS Clade 6, which tend to be high-temperature tolerant and either inbreeding or sterile. Mycological Research 107: 277–290.

Reeser PW, Sutton W, Hansen EM, Remigi P, Adams GC (2011) Phytophthora species in forest streams in Oregon and Alaska. Mycologia 103: 22-35.

Fact sheet authors

Treena Burgess, Ph.D., Centre for Phytophthora Science and Management, School of Biological Sciences and Biotechnology, Murdoch University, Australia; Z. Gloria Abad, Ph.D., USDA-APHIS-PPQ-S&T Beltsville Laboratory, United States of America