Phytophthora amnicola

Name and publication

Phytophthora amnicola Burgess and Jung (2012)

Burgess TI, Hüberli D, Hardy GE StJ, Stukely MJC, and Jung T. 2012. Phytophthora amnicola T. I. Burgess & T. Jung, sp. nov. Persoonia 28: 140–141.

Corresponding author tburgess@murdoch.edu.au

Nomenclature

from Burgess et al. (2012)

Mycobank

MB563849

Etymology

named for the riverside habitat of this species

Typification

Type: WESTERN AUSTRALIA, Perth, Poison Gully Creek, baited from still water, Dec. 2009, D. Hüberli, holotype MURU 471

Ex-type: cultures ex-type CBS 131652 = DH228

Sequences for Ex-type in original manuscript

CBS 131652 = DH228 = ITS JQ029956, β-tubulin JQ029952, HSP90 JQ029944, cox1 JQ029948, NADH JQ029940, and LSU JX069838

Ex-type in other collections

CBS 131652 = DH228 = P19862 (WPC), CPHST-BL 100 (Abad), 61G6 (Yang)

Molecular identification

Voucher sequences for barcoding genes (ITS rDNA and COI) of the ex-type (see Molecular protocols page)

Phytophthora amnicola isolate DH228 ITS rDNA JQ029956

Phytophthora amnicola isolate CPHST BL 100 (= P19862 WPC) COI MH477740

Sequences for ex-type in other sources
P​osition in ITS phylogenetic tree

Clade 6b

Morphological identification

Colonies and cardinal temperatures

Colony morphology is stellate with limited aerial mycelium on V8 agar, rosaceous on carrot agar, and growth on potato-dextrose agar is very slow. Minimum growth temperature 7.5°C, optimum 25–32.5°C, and maximum 37.5°C.

Conditions for growth and sporulation

Sporangia are produced in water cultures (soil extract or river water) and not observed in solid media. Oogonia are not observed in single culture or when paired with tester strains.

Asexual phase

Sporangia are nonpapillate, persistent, ovoid, or limoniform with average dimensions of 62 ± 9.0 × 35.3 ± 5.6 μm (overall range 39–78 × 17–43 μm). Sporangiophores in simple sympodia and internal proliferation, both nested and extended, occur in chains. Hyphal swellings are ellipsoid to irregular, catenulate occurring in clusters. Chlamydospores absent

Sexual phase

Sterile in culture.

Most typical characters

Phytophthora amnicola is in a species cluster with Phytophthora litoralis, Phytophthora fluvialis, Phytophthora moyootj, and Phytophthora thermophila, all recovered from waterways in Western Australia. There are few features to distinguish these species, however P. amnicola is the only species that produces rosaceous colonies on CA.

Specimen(s) evaluated

Australia, Western Australia, Perth, Poison Gully Creek, baited from still water, Dec. 2009, D. Hüberli, CBS131652 = DH228; Lake Jualbup, DH013; Canning River, DH237; Pemberton, 2008, VHS19503

CPHST-BL 100 (ET) = P19862 World Phytophthora Collection – California, USA

Hosts and distribution

Distribution: Western Australia
Substrate: water
Disease note: No pathogenicity trials have been conducted. The species has been recovered from dying blackberry corms, but pathogenicity is not known.
Hosts: known only from water

Retrieved January 18, 2018 from U.S. National Fungus Collections Nomenclature Database.

Additional references and links

Crous PW, Summerell BA, Shivas RG, Burgess TI, Decock CA, Dreyer LL, Granke LL, Guest DI, Hardy GESJ, Hausbeck MK, Hüberli D, Jung T, Koukol O, Lennox CL, Liew ECY, Lombard L, McTaggart AR, Pryke JS, Roets F, Saude C, Shuttleworth LA, Stukely MJC, Vánky K, Webster BJ, Windstam ST, and Groenewald JZ. 2012. Fungal Planet description sheets: 107–127. Persoonia 28: 138–182.

Jung T, Stukely MJC, Hardy GEStJ, White D, Paap T, and Burgess TI. 2011. Multiple new Phytophthora species from ITS Clade 6 associated with natural ecosystems in Australia: evolutionary and ecological implications. Persoonia 26: 13–39.

Hüberli D, Hardy GESJ, White D, Williams N, and Burgess TI. 2013. Fishing for Phytophthora from Western Australia's waterways: A distribution and diversity survey. Australasian Plant Pathology 42: 251–260

Fact sheet authors

Treena Burgess, Ph.D., Centre for Phytophthora Science and Management, School of Biological Sciences and Biotechnology, Murdoch University, Australia; Z. Gloria Abad, Ph.D., USDA-APHIS-PPQ-S&T Beltsville Laboratory, United States of America