Phytophthora polonica

Name and publication

Phytophthora polonica Belbahri, Moralejo & Lefort (2006)

Belbahri L, Moralejo E, Calmin G, Oszako T, Garci­a, JA, Descals E, Lefort F. 2006. Phytophthora polonica, a new species isolated from declining Alnus glutinosa in Poland. FEMS Microbiology Letters 261: 165–174. 

Corresponding author: lassaad.belbahri@etat.ge.ch

Nomenclature

from Belbahri et al. (2006)

Mycobank

MB501096

Etymology

refers to the country where it was isolated

Typification

Type: POLAND, Kolo, isolated from rhizosphere of Alnus glutinosa on July 2004 by T. Oszako; UASWS0198 dry culture in carrot agar deposited in “Herbarium Universitatis Helvaticae Occidentalis”

Ex-type: CBS 119650

Sequences for ex-type in original manuscript

Phytophthora polonica UASWS0198 = ITS rDNA DQ396410, β-Tubulin: DQ399844, translation elongation factor (TEF-1a): DQ399850, NADH dehydrogenase subunit I (NADH) (Kroon et al 2004): DQ399847 (in NCBI as P131445)

Ex-type in other collections

CBS 119650 (= UASWS0198) = P131445 (not WPC code), P19522 (WPC), CPHST BL 109 (Abad)

Molecular identification

Voucher sequences for barcoding genes (ITS rDNA and COI) of the ex-type (see Molecular protocols page)

Phytophthora polonica isolate UASWS0198 = ITS rDNA DQ396410
Phytophthora polonica isolate CPHST BL 109 (= P19522 WPC) COI MH136960

Sequences for ex-type in other sources

NOTE: There is some variety in how the code is cited in the above databases. Q-bank: CBS 119650 (= UASWS189 = UASWS0189 = P13445 not WPC code); Q-bank and WPC as P13445, NCBI as P131445; Q-bank and WPC as UASWS0189 = original manuscript as UASWS0198).

Position in ITS phylogenetic tree

Clade 9b

Morphological identification

adapted from Belhari et al. (2009)

Colonies and cardinal temperatures

Colony morphology on PDA, V8A, MEA with rosette pattern. Minimum growth temperature 6°C, optimum 24–27°C, and maximum 33°C.

Conditions for growth and sporulation

Sporangia not formed in culture media. Chlamydospores and hyphal swellings formed in agar or in soil water extract.

Asexual phase

Sporangia nonpapillate; persistent; mostly ovoid to obpyriform (37–67 L x 31–48 W µm), proliferating internally, often nested or catenulate; originated on long unbranched sporangiophores. Hyphal swellings globose or irregularly shaped, frequently catenulate, toruloid or single, intercalary, or lateral. Chlamydospores abundant spherical, subglobose or pyriform (16–69 µm), intercalary, lateral, or terminal.

Sexual phase

Homothallic. Oogonia smooth-walled; spherical to subglobose (28–48 µm diam); antheridia predominantly paragynous and sometimes amphigynous, mostly bell-shaped to irregularly shaped, less frequently globose or cylindrical; oospores aplerotic to nearly plerotic (27–44 µm diam). A high proportion of aborted oogonia are observed.

Most typical characters

Phytophthora polonica is characterized by the presence of hyphal swellings and chlamydospores and the shape of the antheridia.

Additional specimen(s) evaluated

Phytophthora polonica ex-type CPHST BL 109 = P19522 (World Phytophthora Collection) 

Hosts and distribution

Distribution: Europe (Poland), North America (USA: CT)
Substrate: soil, water
Disease note: slight disease on Alnus twigs in experiments
Host: Alnus glutinosa (Betulaceae)

Retrieved February 01, 2018 from U.S. National Fungus Collections Nomenclature Database.

Additional info:
Substrate: rhizosphere

Additional references and links

Fact sheet author

Z. Gloria Abad, Ph.D., USDA-APHIS-PPQ-S&T Beltsville Laboratory, United States of America