Phytophthora pinifolia

Name and publication

Phytophthora pinifolia Durán, Gryzenh. & Wingf. (2008)

Durán A, Gryzenhout M, Slippers B, Ahumada R, Rotella A, Flores F, Wingfield BD, and Wingfield MJ. 2008. Phytophthora pinifolia sp. nov. associated with a serious needle disease of Pinus radiata in Chile. Plant Pathol. 57: 715–727.

Corresponding author: alvaro.duran@fabi.up.ac.za

Nomenclature

from Durán et al. (2008)

Mycobank

MB51870

Etymology

‘Pinifolia’ refers to the occurrence of the organism on the needles of Pinus radiata.

Typification

Type: CHILE isolated from Pinus radiata in Arauco province, Arauco, Llico plantation, July 2007 by M.J. Wingfield; holotype: PREM 59887

Ex-type: CBS 122924, P16100, CMW 26668

Sequences for ex-type in original manuscript: CMW 26668 = ITS = EU725806, CoxII EU725811 (Martin and Tooley FM35 & FM58)

Ex-type in other collections

CBS 122924, P16100 (WOC/WPC) = CPHST BL 49 (Abad), 47H1 (Yang)

Molecular identification

Voucher sequences for barcoding genes (ITS rDNA and COI) of the ex-type (see Molecular protocols page)

Phytophthora pinifolia isolate CPHST BL 49 (= P16100 WPC) = ITS rDNA MG865566, COI MH136958

Sequences for ex-type in other sources
Position in ITS phylogenetic tree

Clade 6b

Morphological identification

adapted from Durán et al. (2008)

Colonies and cardinal temperatures

Colony morphology after 7 days of growth on V8 agar, potato dextrose agar, and malt extract agar with no distinctive pattern. Minimum temperature for growth is xx°C, optimum xx°C, and maximum xx°C. 

Conditions for growth and sporulation

Sporangia produced abundantly in soil extract water and rare in culture.

Asexual phase

Sporangia nonpapillate; persistent; globose to ovoid, obpyriform (39–61µm L × 27–45 μm W); ocassionally germinating directly; sporangia produced in unbranched sporangiophores. Hyphal swellings spherically radiating. Chlamydospores absent.

Sexual phase

Sterile.

Additional specimen(s) evaluated

Phytophthora pinifolia, ex-type CPHST BL 49, duplicate of P16100 (World Phytophthora Collection)

Hosts and distribution

Distribution: South America (Chile)
Substrate: needles, shoots
Disease note: downy mildew, needle disease, stem necrosis
Host: Pinus radiata (Pinaceae)

Retrieved February 01, 2018 from U.S. National Fungus Collections Nomenclature Database.

Quarantine status

USA: This species was listed as a species of concern during the 2009 Phytophthora prioritization project conducted by USDA APHIS PPQ CPHST PERAL (Schwartzburg et al.).

EPPO alert list 12.2012: Phytophthora pinifolia; panel review date 03.2012 (previously listed pests are at the bottom of this page)

Additional references and links

Fact sheet author

Z. Gloria Abad, Ph.D., USDA-APHIS-PPQ-S&T Beltsville Laboratory, United States of America