Phytophthora nicotianae (to be redescribed 2022)

Name and publication

Phytophthora nicotianae Breda de Haan (1896)

Breda de Haan van. 1896. De bibitziekte in de Deli-tabak veroorzaakt door Phytophthora nicotianae. Meded. uit 's Lands Plantentuin Coll. XV (met plaat) Batavia 15: 1–107 (pg 57).

Nomenclature

Mycobank

MB194443

Synonymy

Phloeophthora nicotianae (Breda de Haan) G.W. Wilson, Mycologia 6 (2): 80 (1914) [MB#246601]
Phytophthora parasitica var. nicotianae (Breda de Haan) Tucker, Research Bulletin of the Missouri Agricultural Experiment Station 153: 173 (1931) [MB#253828]
= Phytophthora manoana Sideris [MB#122828]
= Phytophthora melongenae var. ananaphthoros Sideris [MB#494136]
= Phytophthora parasitica Dastur, Memoirs of the Department of Agriculture India 5 (4): 177 (1913) [MB#194284]
= Phytophthora melongenae Sawada, Special Report Agric. Exp. Stat. (Taiwan): 77 (1915) [MB#232247]
= Phytophthora terrestris Sherb., Phytopathology 7: 127 (1917) [MB#194200]
= Phytophthora parasitica var. rhei G.H. Godfrey, J. Agric. Res.: 21 (1923) [MB#273614]
= Phytophthora tabaci Sawada, Report of the Department of Agriculture Government Research Institute of Formosa 27: 35 (1927) [MB#279692]

Typification

from Breda de Haan (1896)

Type: SUMATRA (island in western Indonesia) Java, Borneo in leaves of Nicotiana tabaccum

Ex-type: LOST

Well-authenticated specimen(s) selected by Gloria Abad: 

  • Selected specimen #1: CPHST BL 44 = P7661 (WPC) (A1) (Indonesia, Nicotiana tabacum)
  • Selected specimen #2: CPHST BL 162 = P6303 (WPC) (A2) (Indonesia, Gramatophyllum sp.)
  • Selected specimen #3: CPHST BL 161 = P1753 (WPC) (A1) (Australia, Nicotiana tabacum)
Selected specimen #1 in other collections:

P7661 (WOC/WPC) = CPHST BL 44 (Abad), IMI 329725

Molecular identification

Voucher sequences for barcoding genes (ITS rDNA and COI) of selected specimens (see Molecular protocols page)

Phytophthora nicotianae isolate CPHST BL 44 (= P7661 WPC) = ITS rDNA MG865550, COI MH136943

Sequences for selected specimen(s) in other sources
  • NCBI: Phytophthora nicotianae CPHST BL 44
  • Phytophthora Database: Phytophthora nicotianae
  • EPPO-Q-bank: Phytophthora nicotianae
  • BOLDSYSTEMS: Phytophthora nicotianae (barcoding COI & ITS)
Position in ITS phylogenetic tree

Clade 1

Morphological identification

Colonies and cardinal temperatures

Colony morphology on V-8 agar and potato dextrose agar with no distinct pattern with coralloid edges, and malt extract agar with slow growth. Minimum temperature for growth 9°C, optimum 24°C, maximum 33°C.

Conditions for growth and sporulation
Asexual phase

Sporangia papillate; persistent and caducous with short pedicels (1–4 µm); ovoid, globose, obovoid, obpyriform, and with irregular shapes (15–64 L x 11–47 W µm), some sporangia with elongated necks and tapered bases; sporangia originated in simple sympodial sporangiophores. Hyphal swellings globose, subglobose, intercalary, many times with radiating hyphae. Chlamydospores globose (15–43 µm), lateral, terminal, intercalary.

Sexual phase

Heterothallic. Oogonia smooth-walled (24–32 µm diam.); antheridia amphyginous (9–21 x 10–15 µm); oospores predominantly aplerotic or slightly aplerotic (19–27 µm diam.).

Most typical characters

Phytophthora nicotianae is characterized by the asexual stage including the sporangia, hyphal swellings, and chlamydospores, and the typical coralloid hyphae observed in culture media.

Specimen(s) evaluated

Phytophthora nicotianae CPHST BL 44 = P7661 (World Phytophthora Collection)

Hosts and distribution

Distribution: cosmopolitan
Substrate: roots, stems, trunk, leaves, fruits, pods
Disease note: black shank of tobacco, damping-off, crown rot, leaf blight, fruit rot. See Erwin & Ribeiro for detailed disease notes by host.
Host: wide host range; 255 genera in 90 families

Retrieved January 31, 2018 from U.S. National Fungus Collections Nomenclature Database.

Additional Info:
Distribution:
USA (CA, HI, TN), Bulgaria, Japan, Taiwan

Additional references and links

Fact sheet author

Z. Gloria Abad, Ph.D., USDA-APHIS-PPQ-S&T Beltsville Laboratory, United States of America