Phytophthora multivora

Name and publication

Phytophthora multivora Scott & Jung (2009)

Scott PM, Burgess TI, Barber PA, Shearer BL, Stukely MJC, Hardy GE StJ, and Jung T. 2009. Phytophthora multivora sp. nov., a new species recovered from declining Eucalyptus, Banksia, Agonis, and other plant species in Western Australia. Persoonia 22: 1–13.

Corresponding author: G.Hardy@Murdoch.edu.au

Nomenclature

from Scott et al (2009)

Mycobank

MB512497

Synonym

referred to as P. sp. 4 by Burgess et al. 2009

Etymology

refers to the wide host range (multi L. = many, -vora L. = feeding)

Typification

Type: AUSTRALIA, collected in Western Australia, Yalgorup, from rhizosphere soil of declining Eucalyptus marginata, May 2007 by P. Scott & T. Jung MURU 434 (dried culture on V8A, Herbarium of Murdoch University, Western Australia)

Ex-type: CBS 124094 = WAC 13201

Sequences for ex-type in original manuscript: CBS 124094 = ITS FJ237521, coxI FJ237508, β-tubulin FJ665260

Ex-type in other collections

CBS 124094 = P19594 (WOC/WPC), CPHST BL 104 (Abad), 55C5 (Yang)

Molecular identification

Voucher sequences for barcoding genes (ITS rDNA and COI) of the ex-type (see Molecular protocols page)

Phytophthora multivora ex-type isolate CPHST BL 104 (= P19594 WPC) = ITS rDNA MG865546, COI MH136939

Phytophthora multivora selected specimen isolate CPHST BL 26 (= P19568 WPC) = ITS rDNA MG865545, COI MH136938

Sequences for ex-type in other sources
Position in ITS phylogenetic tree

Clade 2c

Morphological identification

Colonies and cardinal temperatures

Colony morphology is stellate on V8A and MEA, and petaloid and felty to fluffy on PDA. Minimum growth temperature 5°C, optimum 25°C, and maximum 32.5°C.

Conditions for growth and sporulation

Sporangia are produced in water cultures (soil extract or river water) and not observed in solid media. Oogonia are formed readily in single-strain culture on CA and V8A after about 7 d.

Asexual phase

Sporangia are semipapillate, persistent, and ovoid, limoniform, or ellipsoid in shape. Sporangia with two or three papillae are common. Sporangia average 51.0 ± 10.4 × 30.0 ± 5.1 µm (overall range of 36–58 × 13–33 µm). Sporangiophores in simple sympodia, but occasional external proliferation can result in lax sympodia. Hyphal swellings absent; Chlamydospores absent.

Sexual phase

Homothallic. Oogonia are globose with smooth walls, average size 26.5 ± 1.9 µm (overall range 19–37 µm). Oospores are nearly plerotic, globose with thick walls, average size 23.6 ± 1.8 µm (17.3–33.1). Antheridia paragynous.

Most typical characters

Phytopthora multivora is part of a complex that includes P. citricola, P. plurivora, P. pini, P. acerina, and P. pachypleura. Species in this complex have very similar morphological features.

Specimen(s) evaluated

Australia, Western Australia, Yalgorup, from rhizosphere soil of declining Eucalyptus marginata, May 2007, P. Scott & T. Jung, WAC 13201 = CBS 124094; WAC 13205; from rhizosphere of declining Eucalyptus gomphocephala, WAC 13200; WAC 13202; WAC 13203; from rhizosphere of Agonis flexuosa, WAC 1320

WPC P19594, CPHST BL 104 (ex-type duplicate) 

WPC P19568, CPHST BL 26 (selected specimen)

Hosts and distribution

Distribution: widespread
Substrate: living leaves, roots
Disease note: leaf spot
Host: wide host range

Retrieved January 31, 2018 from U.S. National Fungus Collections Nomenclature Database.

Additional info
Distribution: Western Australia, South Africa, New Zealand, USA, Europe
Substrate: roots, rhizosphere soil, basal and stem cankers, and shoots of some hosts
Disease note: pathogenic toward Eucalyptus gomphocephala, E. marginata, Agonis flexuosa; implicated in decline of Eucalyptus gomphocephala
Hosts: numerous, including Acacia sp., Adenanthos cuneatus, Agonis flexuosa, Banksia baxteri, Banksia menziesii, Banksia grandis, Banksia littoralis, Banksia attenuata, Banksia prionotes, Banksia media, Banksia formosa, Banksia oreophila, Banksia quercifolia, Banksia seminuda, Banksia speciosa, Billardiera sp., Bossiaea aquifolium, Bossiaea sp., Casuarina obesa, Conospermum stoechadis, Corymbia calophylla, Eucalyptus marginata, Eucalyptus gomphocephala, Gastrolobium spinosum, Grevillea prostrata, Hakea undulata, Isopogon sp., Lambertia echinata, Lambertia inermis, Leucopogon capitellatus, Leucopogon verticillatus, Macrozamia riedlei, Patersonia occidentalis, Persoonia longifolia, Podocarpus drouyniana, Pultenaea reticulata, Stirlingia latifolia, Tremandra stelligera, Xanthorrhoea gracilis, Xanthorrhoea preissii, Xanthorrhoea platyphylla, Xanthorrhoea drummondii

Additional references and links

Fact sheet authors

Treena Burgess, Ph.D., Centre for Phytophthora Science and Management, School of Biological Sciences and Biotechnology, Murdoch University, Australia; Z. Gloria Abad, Ph.D., USDA-APHIS-PPQ-S&T Beltsville Laboratory, United States of America