Phytophthora multivesiculata Ilieva, Man in ‘t Veld, Veenbaas-Rijks & Pieters (1998)

Ilieva E, Man in 't Veld WA, Veenbaas-Rijks W, and Pieters R. 1998. Phytophthora multivesiculata, a new species causing rot in Cymbidium. Eur. J. Plant Pathol. 104: 677–684.

from Ilieva et al. (1998)

Mycobank

MB445238

Typification

Type: NETHERLANDS, Mijdrecht, isolated from leaves of Cymbidium on November, 1995, dry culture CBS 6034 deposited in herbarium Centraalbureau voor Schimmelcultures, Baarn, the Netherlands

Ex-type: CBS 545.96

Ex-type in other collections

CBS 545.96 = PD 95/8679, P10410 (WOC/WPC), CPHST BL 50G (Abad), 29E3 (Yang)

Voucher sequences for barcoding genes (ITS rDNA and COI) of the ex-type (see Molecular protocols page)

Phytophthora multivesiculata isolate CPHST BL 50G (= P10410 WPC) = ITS rDNA MG865544, COI MH136937

Sequences for ex-type in other sources

• NCBI: Phytophthora multivesiculata CPHST BL 50G
• NCBI: Phytophthora multivesiculata P10410
• NCBI: Phytophthora multivesiculata CBS 545.96
• NCBI: Phytophthora multivesiculata CBS545.96
• Phytophthora DATABASE: Phytophthora multivesiculata PD-00001 (= P10410)
• EPPO-Q-bank: Phytophthora multivesiculata CBS 545.96 (= PD 95/8679)
• BOLDSYSTEMS: Phytophthora multivesiculata OOMYA 180-07 [COI-5P:680, ITS:803] = CBS 545.96; OOMYA 533-08 [ITS:2284] = CBS 545.96 (barcoding COI & ITS)

Position in ITS phylogenetic tree

Clade 2

Colonies and cardinal temperatures

Colony morphology after 7 days of growth on V8 agar, potato dextrose agar, and malt extract with no distinct pattern to slightly radiate. Minimum temperature for growth is 6°C, optimum 21–27°C, and maximum 30°C.

Asexual phase

Sporangia nonpapillate, rarely with two apices; persistent; ovoid, obpyriform occasionally with tapered base (30–60 L x 20–41 W μm); sporangia showing internal and external proliferation and produced singly on long, slender, mainly twisted sporangiophores or in sympodial arrangement of 2 or 3 sporangia; some sporangia laterally attached to sporangiophore. Hyphal swellings globose and catenulate. Chlamydospores absent.

Sexual phase

Homothallic. Oogonia smooth-walled (28–50 μm diam.); antheridia predominantly amphigynous, irregularly spherical or ellipsoidal, sometimes with digitated projections; oospores plerotic or slightly aplerotic (24–42 μm diam.).

NOTES:

• Original publication refers to the presence of semipapillate and nonpapillate sporangia. In our observations of the ex-type P10410, only nonpapillate sporangia have been observed.
• Original manuscript refers to oospores mostly aplerotic. Oospores of specimen P10410 are plerotic or slightly aplerotic.

Most typical characters

Phytophthora multivesiculata is characterized by the presence of globose and catenulated hyphal swellings.

Specimen(s) evaluated

Phytophthora multivesiculata ex-type CPHST BL 50G, duplicate of P10410 ex-type (World Phytophthora Collection), which is a duplicate of ex-type CBS 545.96

Distribution: Australia, Europe (the Netherlands, type)
Substrate: leaves, stems, base of bulbs
Disease note: leaf and stem rot, bulb rot
Host: Cymbidium sp. (Orchidaceae)

Retrieved January 31, 2018 from U.S. National Fungus Collections Nomenclature Database.

USA: This species was listed as a species of concern during the 2009 Phytophthora prioritization project conducted by USDA APHIS PPQ CPHST PERAL (Schwartzburg et al.).

• Phytophthora DATABASE: Phytophthora multivesiculata
• EPPO-Q-bank: Phytophthora multivesiculata
• SMML USDA-ARS: Phytophthora multivesiculata
• EPPO Global Database: Phytophthora multivesiculata
• CABI Invasive Species Compendium: Phytophthora multivesiculata
• Encyclopedia of Life (EOL): Phytophthora multivesiculata
• Index Fungorum (IF): Phytophthora multivesiculata
• Plantwise Knowledge Bank: Phytophthora multivesiculata

• Google All Phytophthora multivesiculata
• Google Images Phytophthora multivesiculata
• Google Scholar Phytophthora multivesiculata

Fact sheet authors

Treena Burgess, Ph.D., Centre for Phytophthora Science and Management, School of Biological Sciences and Biotechnology, Murdoch University, Australia; Z. Gloria Abad, Ph.D., USDA-APHIS-PPQ-S&T Beltsville Laboratory, United States of America