Phytophthora arenaria

Name and publication

Phytophthora arenaria Rea, Stukely & Jung (2011)

Rea AJ, Burgess TI, Hardy GE StJ, Stukely MJC, and Jung T. 2011. Two novel and potentially endemic species of Phytophthora associated with episodic dieback of Kwongan vegetation in the south-west of Western Australia. Plant Pathol. 60: 1055–1068.

Corresponding author: tburgess@murdoch.edu.au

Nomenclature

from Rea et al. (2011)

Mycobank

MB518792

Synonymy

referred to as P. sp.1 by Burgess et al. 2009

Etymology

refers to the association of this species with sandy soils

Typification

Type: WESTERN AUSTRALIA, Eneabba, isolated from soil sample collected beneath symptomless Eucalyptus drummondii growing in native Kwongan vegetation, February 2009, T. Jung, MURU 455 (dried culture on V8A in the herbarium of Murdoch University, Western Australia).

Ex-type: CBS 127950 deposited at the Centraalbureau voor Schimmelcultures (CBS) Utrecht, Netherlands

Sequences for ex-type in original manuscript: Phytophthora arenaria CBS 127950: ITS rDNA: HQ013219, COX 1 HQ013203

Ex-type in other collections

CBS 127950 = ENA3 = P19599  (WOC/WPC), CPHST BL 78 (Abad), 55C2 (Yang)

Molecular identification

Voucher sequences for barcoding genes (ITS rDNA and COI) of the ex-type (see Molecular protocols page)

Phytophthora arenaria isolate CPHST BL 78 (= P19599 WPC) = ITS rDNA MG783377, COI MH136848

Sequences for ex-type in other sources
Position in ITS phylogenetic tree

Clade 4

Morphological identification

Colonies and cardinal temperatures

Colony morphology is appressed with no distinctive growth pattern and regular smooth margins on CA, V8A, MEA, and PDA. Minimum growth temperature 10°C, optimum 25°C, and maximum 32.5°C.

Conditions for growth and sporulation

Sporangia are produced in water cultures (soil extract or river water) and not observed in solid media. Oogonia are formed readily in single-strain culture on CA and V8A after about 14 d.

Asexual phase

Sporangia are papillate, persistent, and predominantly ovoid. Sporangia average 31.8 ± 4.6 x 27.3 ± 3.5 mm (overall range 20.2–53.0 x 12.5–35.0 mm). Sporangiophores in simple sympodia with no proliferation. Hyphal swellings are rare. Chlamydospores absent

Sexual phase

Homothallic. Oogonia are globose with wavy walls turning golden-brown on maturity, average size 25.3 ± 2.2 mm (19.6–34.3 mm). Oospores are aplerotic, globose with thick walls, average size 22.3 ± 1.8 µm (16.0–28.3). Antheridia are paragynous.

Most typical characters

Phytopthora arenaria forms a species complex with Phytophthora boodjera and Phytophthora alticola and is morphologically very similar to these species.

Specimen(s) evaluated

Australia; Western Australia: Australia, Eneabba, isolated from soil sample collected beneath asymptomatic Eucalyptus drummondii, February 2009, T. Jung. CBS 127950 = ENA3; CBS 125800 = ENA1; ENA4; from Banksia attenuata, June 2008, VHS 19931; VHS19950; Badgingarra, isolated from Banksia attenuata, April 2006, VHS15453; VHS15489; Bunbury, isolated from Banksia littoralis, February 2002, VHS 10154; Cooljarloo, isolated from Hibbertia hypericoides, January 2009, B. Dunstan, CLJO 142; Kalbarri, from soil beneath symptomatic vegetation, June 1986, DDS 1221; Lancelin, from Banksia menziesii, November 2001, VHS 9861

Additional isolate(s): CPHST BL 78 (ET) = P19599 World Oomycetes /Phytophthora Collection – California, USA

Hosts and distribution

Distribution: Western Australia
Substrate: roots, collars, and rhizosphere soil
Disease note: proven pathogenic toward Banksia attenuata
Hosts: Banksia littoralis, B. attenuata, B. sphaerocarpa, B. hookeriana, B. menziesii, Hibbertia hypericoides, Eucalyptus drummondii

Retrieved January 18, 2018 from U.S. National Fungus Collections Nomenclature Database.

Additional references and links

Burgess TI, Webster JL, Ciampini JA, White DW, Hardy GESJ, and Stukely MJC. 2009. Re-evaluation of Phytophthora species isolated during 30 years of vegetation health surveys in Western Australia using molecular techniques. Plant Disease 93, 215–223.

Maseko B, Coutinho TA, Burgess TI, Wingfield BD, and Wingfield MJ. 2007. Two new species of Phytophthora from South African eucalypt plantations. Mycological Research 111: 1321–1338.

Fact sheet authors

Treena Burgess, Ph.D., Centre for Phytophthora Science and Management, School of Biological Sciences and Biotechnology, Murdoch University, Australia; Z. Gloria Abad, Ph.D., USDA-APHIS-PPQ-S&T Beltsville Laboratory, United States of America