Phytophthora tenuimura

Name and publication

Phytophthora tenuimura T. Jung, T. Corcobado, T. Májek & M. Ferreira, (2022)

Jung T, Milenković I, Corcobado T. et al. 2022. Extensive morphological and behavioural diversity among fourteen new and seven described species in Phytophthora Clade 10 and its evolutionary implications. Persoonia-Molecular Phylogeny and Evolution of Fungi 49: 1–57.

Nomenclature

Mycobank

MB842945

Etymology

Name refers to the thin oospore walls (tenuis Lat. = thin; mura Lat. = wall).

Typification

Type: USA, Louisiana, Archie, isolated from a naturally fallen leaf in a flooded swamp forest. March 2020, T. Corcobado & T. Májek, holotype HNHM-MYC-009709, dried culture on V8A, Herbarium of the Hungarian Natural History Museum, Budapest, Hungary

Ex-type: culture CBS 149227 = NRRL 64142 = LU052

Sequences for ex-type in original manuscript:  LSU ON000704, ITS ON000987, β-tubulin OM975977, hsp90 OM976494, tigA OM974671, rpl10 OM97531, tef-1a OM984957, enolase OM976588, cox1 ON013864, ypt1 ON024514, nadh1 OM976973, rps10 OM976732

Molecular identification

Position in ITS phylogenetic tree

Clade 10a

Morphological identification

Colonies and cardinal temperatures

Colony morphology with limited aerial mycelium on V8A and CA with a radiate pattern on V8A and a chrysanthemum pattern on CA; colonies on PDA were dense-felty and stoloniferous. Optimum growth temperature 27.5°C and maximum 30°C.

Conditions for growth and sporulation

Sporangia produced in non-sterile soil extract after 24–36h. Oogonia produced readily in single culture on V8A.

Asexual phase

Sporangia were nonpapillate, persistent, and predominantly ovoid to elongated ovoid and ellipsoid to elongated ellipsoid in shape. Sporangia averaged 47.5 x 25.0 µm (overall range 30.6–84.9 x 15.6–30.4 µm), proliferation internal, nested, and extended. Sporangiophores unbranched. Hyphae undulating; hyphal swellings absent.Chlamydospores absent.

Sexual phase

Homothallic. Oogonia smooth or wavy walls, 61–61 µm. Oospores plerotic, thin-walled, size ranged 24–54 µm. Antheridia paragynous.

Most typical characters

Resides in phylogenetic clade 10d, with two other species all recovered from fallen leaves in swamp forest, Louisiana, USA. Unlike P. procera and P. ludoviciana, P. tenuimura produces oospores in culture.

Additional specimen(s) evaluated

USA, Louisiana, Archie, isolated from a naturally fallen leaf in a flooded swamp forest. March 2020, T. Corcobado & T. Májek, LU050, LU051, LU065, LU066, LU073, LU074

Hosts and distribution

Distribution: USA
Substrate: fallen leaf
Disease note: no known disease
Host: no known host

Additional references and links

none available

Fact sheet author

Treena Burgess, Ph.D., Phytophthora Science and Management, Harry Butler Institute, Murdoch University