Phytophthora pseudokernoviae

Name and publication

Phytophthora pseudokernoviae T. Jung, M. Horta Jung, A. Durán & E. Sanfuentes (2022)

Jung T, Milenković I, Corcobado T. et al. 2022. Extensive morphological and behavioural diversity among fourteen new and seven described species in Phytophthora Clade 10 and its evolutionary implications. Persoonia-Molecular Phylogeny and Evolution of Fungi 49: 1–57.

Nomenclature

Mycobank

MB843149

Etymology

Name refers to the morphological similarity to P. kernoviae.

Typification

Type: Chile, Parque Oncol, isolated from a naturally fallen necrotic leaf of Drimys winteri in a Valdivian rainforest,, Nov. 2014, T. Jung, A. Durán & E. Sanfuentes, holotype HNHM-MYC-009707, dried culture on V8A, Herbarium of the Hungarian Natural History Museum, Budapest

Ex-type: culture CBS 148796 = NRRL 64351 = CL012

Sequences for ex-type in original manuscript:  LSU ON000686, ITS ON000780, β-tubulin OM975959, hsp90 OM976476, tigA OM974653, rpl10 OM974513, tef-1a OM984939, enolase OM976570, cox1 ON013846, ypt1 ON024996, nadh1 OM976955, rps10 OM976714

Molecular identification

Position in ITS phylogenetic tree

Clade 10a

Morphological identification

Colonies and cardinal temperatures

Colony morphology appressed uniform to faintly striate on V8A and faintly radiate on CA, dense-woolly and faintly petaloid on PDA. Optimum growth temperature 15°C and maximum 25°C.

Conditions for growth and sporulation

Sporangia produced in non-sterile soil extract after 1–2d. Oogonia produced readily in single culture on V8A after 2–3 weeks.

Asexual phase

Sporangia were papillate, caducous, and predominantly ovoid in shape. Sporangia averaged 42.7 x 29.7 µm (overall range 30.5–55.0 x 21.3–35.7 µm), external proliferation. Sporangiophores dense sympodia. Hyphal swellings rare. Chlamydospores absent.

Sexual phase

Homothallic. Oogonia smooth-walled mostly with a tapering base turning golden brown with age, 19–39 µm. Oospores predominantly plerotic, size ranged 16–36 µm. Antheridia amphigynous.

Most typical characters

Resides in phylogenetic clade 10a and is closely related to P. chilensis, P. kernoviae and P. pseudochilensis. These species have similar morphology and low temperature optima and maxima.

Additional specimen(s) evaluated

Chile, Parque Oncol, isolated from a naturally fallen necrotic leaf of D. winteri in a Valdivian rainforest, Nov. 2014, T. Jung, A. Durán & E. Sanfuentes, CL013; isolated from a stream running through a Valdivian rainforest using a Nothofagus obliqua leaf as bait, CL156.

Hosts and distribution

Distribution: Chile
Substrate: fallen leaves and forest stream
Disease note: no known disease
Host: no known host

Additional references and links

none available

Fact sheet author

Treena Burgess, Ph.D., Phytophthora Science and Management, Harry Butler Institute, Murdoch University