Phytophthora pseudochilensis

Name and publication

Phytophthora pseudochilensis T. Jung, M. Horta Jung, E. Sanfuentes & I. Milenković (2022)

Jung T, Milenković I, Corcobado T. et al. 2022. Extensive morphological and behavioural diversity among fourteen new and seven described species in Phytophthora Clade 10 and its evolutionary implications. Persoonia-Molecular Phylogeny and Evolution of Fungi.49: 1– 57.

Nomenclature

Mycobank

MB843147

Etymology

Name refers to the morphological similarity to P. chilensis.

Typification

Type: Chile, Parque Oncol, isolated from a stream running through a Valdivian rainforest using a Rhododendron leaf as bait, Nov. 2014, T. Jung, A. Durán & E. Sanfuentes, holotype HNHM-MYC-009705, dried culture on V8A, Herbarium of the Hungarian Natural History Museum, Budapest

Ex-type: culture CBS 148798 = NRRL 64352 = CL168

Sequences for ex-type in original manuscript:  LSU ON000679, ITS ON000773, β-tubulin OM975952, hsp90 OM976469, tigA OM974646, rpl10 OM974506, tef-1a OM984932, enolase OM976563, cox1 ON013839, ypt1 ON024989, nadh1 OM976948, rps10 OM976707

Molecular identification

Position in ITS phylogenetic tree

Clade 10a

Morphological identification

Colonies and cardinal temperatures

Colony morphology appressed with a dendroid to stoloniferous pattern with irregular margins on V8A, uniform and appressed with limited aerial mycelium on CA, and dense-felty with irregular raised lobes separated by irregular trenches on PDA. Optimum growth temperature 15°C and maximum 25°C.

Conditions for growth and sporulation

Sporangia produced in non-sterile soil extract after 1–2d. Oogonia produced readily in single culture on V8A after 2–3 weeks.

Asexual phase

Sporangia were papillate, caducous, and predominantly ovoid to elongated ovoid in shape. Sporangia averaged 48.8 x 27 µm (overall range 25.6–69.1 x 17.4–39.4 µm), external proliferation. Sporangiophores dense sympodia. Hyphal swellings rare. Chlamydospores absent.

Sexual phase

Homothallic. Oogonia smooth-walled mostly with a tapering base, 16–35 µm. Oospores predominantly plerotic, size ranged 15–32 µm, high abortion rate. Antheridia amphigynous.

Most typical characters

Resides in phylogenetic clade 10a and is closely related to P. chilensis, P. kernoviae, and P. pseudokernoviae. These species have similar morphology and low temperature optima and maxima.

Additional specimen(s) evaluated

Chile, Parque Oncol, isolated from streams running through Valdivian rainforests using Rhododendron leaves as baits, Nov. 2014, T. Jung, A. Durán & E. Sanfuentes, CL335, CL336, CL337, CL338, CL339.

Hosts and distribution

Distribution: Chile
Substrate: forest stream
Disease note: no known disease
Host: no known host

Additional references and links

none available

Fact sheet author

Treena Burgess, Ph.D., Phytophthora Science and Management, Harry Butler Institute, Murdoch University