Phytophthora multiglobulosa

Name and publication

Phytophthora multiglobulosa T. Jung, M. Junaid, M. Horta Jung & I. Milenković (2022)

Jung T, Milenković I, Corcobado T. et al. 2022. Extensive morphological and behavioural diversity among fourteen new and seven described species in Phytophthora Clade 10 and its evolutionary implications. Persoonia-Molecular Phylogeny and Evolution of Fungi 49: 1–57.

Nomenclature

Mycobank

MB843003

Etymology

Name refers to the presence of multiple lipid globules in many oospores.

Typification

Type: Indonesia, South Sulawesi Province, District of North Toraja, isolated from a naturally fallen leaf floating in a stream running through a tropical hill rainforest, May 2019, T. Jung, M. Junaid & N. Nasri, holotype HNHMMYC- 021539,, dried culture on V8A, Herbarium of the Hungarian Natural History Museum, Budapest

Ex-type: culture CBS 148799 = SL005

Sequences for ex-type in original manuscript:  LSU ON000669, ITS ON000763, β-tubulin OM975942, hsp90 OM976459, tigA OM974636, rpl10 OM974496, tef-1a OM984922, enolase OM976554, cox1 ON013829, ypt1 ON024979, nadh1 OM976939, rps10 OM976697

Molecular identification

Position in ITS phylogenetic tree

Clade 10a

Morphological identification

Colonies and cardinal temperatures

Colony morphology submerged to appressed colonies with a radiate pattern and irregular margins on V8A and CA, and dense-woolly and uniform on PDA. Optimum growth temperature 20°C and maximum 27.5°C.

Conditions for growth and sporulation

Sporangia produced in non-sterile soil extract after 1–2d. Oogonia produced readily in single culture on V8A after 2–3 weeks.

Asexual phase

Sporangia were papillate, caducous, and predominantly ovoid and limoniform in shape. Sporangia averaged 43.1 x 27.1 µm (overall range 28.3–64.9 x 21.1–36.5 µm), external proliferation. Sporangiophores dense sympodia. Hyphal swellings absent. Chlamydospores absent.

Sexual phase

Homothallic. Oogonia smooth-walled, 17–38 µm. Oospores plerotic containing multiple lipid globules, size ranged 15–35 µm. Antheridia amphigynous.

Most typical characters

Resides in phylogenetic clade 10a and is closely related to P. celebensis and P. javanensis. These species have similar morphology and have to date been recovered only from tropical rainforests streams in Indonesia.

Additional specimen(s) evaluated

Indonesia, South Sulawesi Province, District of North Toraja, isolated from naturally fallen leaves floating in a stream running through a tropical hill rainforest, May 2019, T. Jung, M. Junaid & N. Nasri, SL006, SL007

Hosts and distribution

Distribution: Indonesia
Substrate: fallen leaves in forest stream
Disease note: no known disease
Host: no known host

Additional references and links

none available

Fact sheet author

Treena Burgess, Ph.D., Phytophthora Science and Management, Harry Butler Institute, Murdoch University