Phytophthora kwongonina Burgess (2018)

Burgess TI, Simamora AV, White D, Wiliams B, Schwager M, Stukely MJC, and Hardy GE StJ. 2018. New species from Phytophthora Clade 6a: evidence for recent radiation. Persoonia 41: 1–17.

Corresponding author: tburgess@murdoch.edu.au

from Burgess et al. (2018)

Mycobank

MB822012

Etymology

refers to association with the kwongon vegetation in the southwest of Western Australia

Typification

Type: AUSTRALIA, Western Australia, Bunbury, from rhizosphere soil of dying Banksia grandis, isolated by the VHS, 2010 (holotype MURU 477, dried culture on V8A, Herbarium of Murdoch University, Western Australia)

Ex-type: CBS 143060 and VHS 23298

Sequences for ex-type in original manuscript: Phytophthora kwongonina strain VHS 23298 = ITS JN547636, TUB MF326824, HSP MF326876, COX MF326847 and NADH MF326914

Ex-type in other collections

CBS 143060 = VHS 23298 = CPHST BL 187 (Abad)

Voucher sequences for barcoding genes (ITS rDNA and COI) of the ex-type (see Molecular protocols page)

Phytophthora kwongonina isolate VHS 23298 ITS rDNA JN547636

Phytophthora kwongonina isolate CPHST BL 187 COI MH477747

Sequences for ex-type in other sources

• NCBI: Phytophthora kwongonina CPHST BL 187
• NCBI: Phytophthora kwongonina VHS 23298
• Phytophthora DATABASE: Phytophthora kwongonina 
• EPPO-Q-bank: Phytophthora kwongonina 
• BOLDSYSTEMS: Phytophthora kwongonina 

Position in ITS phylogenetic tree

Clade 6a

Colonies and cardinal temperatures

Colony morphology on V8 agar, CA, and PDA was cottony with a slight petaloid pattern, growth was appressed with striations on MEA. Minimum growth temperature 4°C, optimum 25°C, and maximum 35°C.

Conditions for growth and sporulation

Sporangia are produced in water cultures (soil extract or river water) and not observed in solid media. Oogonia are formed readily in single-strain culture on CA and V8 after about 14 d.

Asexual phase

Sporangia were nonpapillate, persistent, and predominantly ovoid to elongated ovoid in shape. Sporangia averaged 57.5 ± 11.2 x 36.0 ± 6.9 µm (overall range 34.6–87.0 x 23.2–56.5 µm). Sporangiophores were simple with internal proliferation both nested and extended. Hyphal swellings were predominantly spherical, 12.2–46.4 µm in diameter. Chlamydospores absent.

Sexual phase

Homothallic. Oogonia had wavy walls and averaged 45.4 ± 3.4 mm (36.7–52.4 µm). Oospores were highly aplerotic, globose with very think walls, and pale on maturity, average size 37.1 ± 2.9 µm (31.9–44.1). Antheridia are paragynous.

Most typical characters

Phytophthora kwongonina closely resembles P. rosacearum, P. pseudorosacerum, and P. cooljarloo. Its most distinguishing characteristic are the spherical hyphal swellings with radiating hyphae which appear like small chlamydospores except that the wall doesn’t form.

Specimen(s) evaluated

Australia, Western Australia, Bunbury, from rhizosphere soil of dying Banksia grandis, isolated by the VHS, 2010, CBS 143060 = VHS 23298; Cervantes, from rhizosphere soil of dying Banksia prionotes, TC Hill, 1986, TCH009; Fitzgerald River National Park, from rhizosphere soil of dying Xanthorrhoea platyphylla, isolated by the VHS, 1993, DDS 3599

NOT FOUND as of June 22, 2018 in U.S. National Fungus Collections Nomenclature Database.

• Phytophthora DATABASE: Phytophthora kwongonina 
• EPPO-Q-bank: Phytophthora kwongonina 
• SMML USDA-ARS: Phytophthora kwongonina 
• EPPO Global Database: Phytophthora kwongonina 
• CABI Invasive Species Compendium: Phytophthora kwongonina 
• Encyclopedia of Life (EOL): Phytophthora kwongonina 
• Index Fungorum (IF): Phytophthora kwongonina 
• Plantwise Knowledge Bank: Phytophthora kwongonina 

• Google All Phytophthora kwongonina
• Google Images Phytophthora kwongonina
• Google Scholar Phytophthora kwongonina

Fact sheet authors

Treena Burgess, Ph.D., Centre for Phytophthora Science and Management, School of Biological Sciences and Biotechnology, Murdoch University, Australia; Z. Gloria Abad, Ph.D., USDA-APHIS-PPQ-S&T Beltsville Laboratory, United States of America