Phytophthora cooljarloo

Name and publication

Phytophthora cooljarloo Burgess (2018) 

Burgess TI, Simamora AV, White D, Wiliams B, Schwager M, Stukely MJC, Hardy GE StJ. 2018. New species from Phytophthora Clade 6a: evidence for recent radiation. Persoonia 41: 1–17.

Corresponding author: ​tburgess@murdoch.edu.au

Nomenclature

from Burgess et al. (2018)

Mycobank

MB822011

Etymology

refers to the location where the isolates were recovered

Typification

Type: AUSTRALIA, Western Australia, Cooljarloo, from rhizosphere soil of dying Hibbertia sp., W.A. Dunstan, 2008 (holotype MURU 479, dried culture on V8A, Herbarium of Murdoch University, Western Australia)

Ex-type: CBS 143062

Sequences for ex-type in original manuscript:  Phytophthora sp. kwongan/TIB-2010 strain CLJO100: ITS HQ012957, HSP HQ012925, COX HQ012881 

Phytophthora cooljarloo strain CLJO100: TUB MF326816, NADH MF326910 

Ex-type in other collections

CBS 143062 = TIB-2010, CLJO100, CPHST BL 185 (Abad)

Molecular identification

Voucher sequences for barcoding genes (ITS rDNA and COI) of the ex-type (see Molecular protocols page)

Phytophthora cooljalroo isolate CLJO100 ITS rDNA HQ012957

Phytophthora cooljalroo isolate CPHST BL 185 COI MH477745

Sequences for ex-type in other sources
Position in ITS phylogenetic tree

Clade 6a

Morphological identification

Colonies and cardinal temperatures

Colony morphology on V8 agar and CA was cottony with a slight petaloid pattern, growth was appressed with striations on MEA, and cottony and rosaceous on PDA. Minimum growth temperature 4°C, optimum 25°C, and maximum 35°C.

Conditions for growth and sporulation

Sporangia are produced in water cultures (soil extract or river water) and not observed in solid media. Oogonia are formed readily in single-strain culture on CA and V8 after about 14 d.

Asexual phase

Sporangia were nonpapillate, persistent, and predominantly ovoid in shape. Sporangia averaged 55.0 ± 9.5 x 37.6 ± 5.5 µm (overall range 30.6–79.1 x 25.1–49.8 µm). Sporangiophores were simple with internal proliferation both nested and extended. Hyphal swellings absent. Chlamydospores absent.

Sexual phase

Homothallic. Oogonia had wavy walls and averaged 41.9 ± 4.0 mm (31.9–48.3 µm). Oospores were aplerotic, globose, with a large ooplast, and pale on maturity, average size 35.1 ± 3.5 µm (26.1–39.9). 80% of oospores aborted after formation of the walls. Antheridia are paragynous.

Most typical characters

Phytophthora cooljarloo closely resembles Phytophthora rosacearum, Phytophthora pseudorosacerum, and Phytophthora kwongonina. Its most distinguishing characteristic is the presence of large laterally attached antheridia.

Specimen(s) evaluated

Australia, Western Australia, Cooljarloo, from rhizosphere soil of dying Hibbertia sp., WA Dunstan, 2008, CBS 143062 = CLJO100; from rhizosphere soil of mixed native vegetation, R Hart, 1996, HSA2313

Hosts and distribution

NOT FOUND as of June 2018 in U.S. National Fungus Collections Nomenclature Database.

Additional references and links

  • Phytophthora DATABASE: Phytophthora cooljarloo
  • EPPO-Q-bank: Phytophthora cooljarloo
  • SMML USDA-ARS: Phytophthora cooljarloo
  • EPPO Global Database: Phytophthora cooljarloo
  • CABI Invasive Species Compendium: Phytophthora cooljarloo
  • Encyclopedia of Life (EOL): Phytophthora cooljarloo 
  • Index Fungorum (IF): Phytophthora cooljarloo
  • Plantwise Knowledge Bank: Phytophthora cooljarloo

Fact sheet authors

Treena Burgess, Ph.D., Centre for Phytophthora Science and Management, School of Biological Sciences and Biotechnology, Murdoch University, Australia; Z. Gloria Abad, Ph.D., USDA-APHIS-PPQ-S&T Beltsville Laboratory, United States of America