Phytophthora lilii

Name and publication

Phytophthora lilii Rahman, Uematsu, Kimishima & Kageyama (2015)

Rahman MZ, Uematsu S, Kimishima E, Kanto T, Kusunoki M, Motohashi K, Ishiguro Y, Suga H, and Kageyama K. 2015. Two plant pathogenic species of Phytophthora associated with stem blight of Easter lily and crown rot of lettuce in Japan. Mycoscience 56: 419–433 (pg. 424).

Corresponding author: rahman@green.gifu-u.ac.jp

Nomenclature

from Rahman et al. (2015)

Mycobank

MB807071

Etymology

named after the host plant, Lilium longiflorum (Easter lily)

Typification

Type: JAPAN, Kagoshima, Okinoerabu Island, in stems of Lilium longiflorum, May 1987, leg. E. Kimishima, NBRC H-13200 (freeze dried)

Ex-type: culture, NBRC 32174, MAFF 237500, CBS 135746

Sequences for ex-type in original manuscript: Phytophthora sp. EL-8701 = 60SL10 AB856779, β-tub AB856782, EF1-a AB856788, Eno AB856791, HSP90 AB856794, LSU AB856797, tigA AB856800, cox 1 AB856785

Ex-type in other collections

CBS 135746 = EL-8701 = P19991 (WOC/WPC), CPHST BL 123 (Abad)

Molecular identification

Voucher sequences for barcoding genes (ITS rDNA and COI) of the ex-type (see Molecular protocols page)

Phytophthora lilii isolate CPHST BL 123 (= P19991 WPC) = ITS rDNA MG865523, COI MH136918

Sequences for ex-type in other sources
Position in ITS phylogenetic tree

Clade X2

Morphological identification

adapted from Rahman et al. (2015)

Colonies and cardinal temperatures

Colony morphology after 7 days of growth on V8 agar, potato dextrose agar, and malt extract agar with no distinctive pattern. Minimum temperature for growth is 5°C, optimum 28°C, and maximum 33°C. 

Conditions for growth and sporulation

Oogonia and oospores produced abundantly in single cultures on V8A and CMA.

Asexual phase

Sporangia nonpapillate; persistent; obpyriform, limoniform, elongated, ellipsoid, ovoid or distorted (av. 50 long x 29 μm wide); terminal with internal (extended and nesting) and external proliferation; borne in simple and unbranched or simple sympodial sporangiophores. Hyphal swellings globose and irregular. Chlamydospores globose (34 μm diam.), thin-walled, intercalary.

Sexual phase

Homothallic. Oogonia smooth, spherical, sub-spherical or funnel-shaped (av. 33 μm diam.); antheridia mostly paragynous, rarely amphigynous, regularly spherical or ovoid ellipsoid in shape, large size equal to oogonium (av. 20 x 15 μm wide); oospores aplerotic, spherical (av. 28 μm diam.).

Most typical characters

Phytophthora lilii is characterized by having hyphal swellings, chlamydospores, internal, nested, and extended proliferation of sporangia with subsequent empty sporangia and external proliferation, funnel-shaped oogonia, mostly paragynous and intercalary large antheridia, unique colony pattern, and high optimal growth temperature.

Additional specimen(s) evaluated

Phytophthora lilii ex-type CPHST BL 123, duplicate of P19991 (World Phytophthora Collection), which is a duplicate of CBS 135746

Hosts and distribution

Distribution: Asia (Japan)
Substrate: stems
Disease note: stem blight
Host: Lilium longiflorum (Liliaceae)

Retrieved January 31, 2018 from U.S. National Fungus Collections Nomenclature Database.

Additional references and links

  • Phytophthora DATABASE: Phytophthora lilii
  • EPPO-Q-bank: Phytophthora lilii
  • SMML USDA-ARS: Phytophthora lilii
  • EPPO Global Database: Not available
  • CABI Invasive Species Compendium: Phytophthora lilii
  • Encyclopedia of Life (EOL): Phytophthora lilii
  • Index Fungorum (IF): Phytophthora lilii
  • Plantwise Knowledge Bank: Phytophthora lilii

Fact sheet author

Z. Gloria Abad, Ph.D., USDA-APHIS-PPQ-S&T Beltsville Laboratory, United States of America