Species of the genus Nodele, subgenus Nodele live in pine forest litter, dried leaves, galleries of bark beetles, or in association with book lice. They are probably general predators. The single species of the subgenus Aztecocheyletus, N. (A.) conquistador has been found in association with the megachilid bee Aztecanthidium tenochtitlanicum in Mexico. This mite species is probably specific to its host since there are several records indicating non-random association. This species probably preys on small invertebrates in the bee nest.